The binding of abrin and ricin by Ehrlich ascites tumor cells.
نویسندگان
چکیده
teins on the tumor cells was shown to be specific, pH de pendent, and concentration dependent. Native ricin, abrin, D-galactose, and its sterically related saccharides inhibit this specific binding, while concanavalin A, bovine serum albumin, heat-denatured abrin or ricin, and other sac charides do not. In the presence of D-galactose, the inhibi tion of protein biosynthesis by ricin does not occur in vitro.
منابع مشابه
The Binding of Abrin and Ricin by Ehrlich Ascites Tumor Cells1
teins on the tumor cells was shown to be specific, pH de pendent, and concentration dependent. Native ricin, abrin, D-galactose, and its sterically related saccharides inhibit this specific binding, while concanavalin A, bovine serum albumin, heat-denatured abrin or ricin, and other sac charides do not. In the presence of D-galactose, the inhibi tion of protein biosynthesis by ricin does not oc...
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As the possible mechanisms of inhibition of protein biosynthesis, it was demonstrated that the toxic proteins abrin and ricin cause the degradation of polyribosomes of rat liver or Ehrlich ascites tumor cells. Abrin and ricin have no direct effect on the structural and functional integrity of polyribosomes but act indirectly by increasing the RNase activity in the postmicrosomal supernatant fra...
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Abrin, ricin, and cisplatin produced significant increases in survival times of mice inoculated with 10(6) Ehrlich ascites carcinoma or L1210 leukemia cells 24 hours prior to treatment. Combinations of abrin or ricin with cisplatin produced markedly synergistic action in prolonging survival times of mice bearing cell line A of L1210 leukemia. For example, a dosage of 1.33 micrograms per kg abri...
متن کاملOn the toxic proteins abrin and ricin. Studies of their binding to and entry into Ehrlich ascites cells.
The mechanism of penetration of the toxic plant proteins abrin and ricin into Ehrlich ascites cells has been studied by measuring, under different experimental conditions, the lag occurring from the time the cells have been incubated with the toxins until protein synthesis in the cells is inhibited. When Ehrllch ascites cells were incubated with abrln or ricin at 37”, a lag time of at least 30 ...
متن کاملEffect of crystalline abrin on the biosynthesis of protein, RNA, and DNA in experimental tumors.
Crystalline abrin isolated from Abrus precatorius L. has been shown to have a strong inhibitory effect on the protein biosynthesis of Ehrlich ascites tumor and Yoshida ascites hepatoma cells and a moderate inhibitory effect on the DNA biosynthesis of these neoplastic cells. It has no significant effects on the RNA biosynthesis and cellular respiration of these neoplastic cells at a much higher ...
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ورودعنوان ژورنال:
- Cancer research
دوره 33 11 شماره
صفحات -
تاریخ انتشار 1973